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2 edition of A comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis found in the catalog.

A comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis

by David Walter Thornbury

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  • 28 Currently reading

Published .
Written in English


Edition Notes

Statementby David Walter Thornbury
The Physical Object
Paginationvi, 61 leaves :
Number of Pages61
ID Numbers
Open LibraryOL24596924M
OCLC/WorldCa6082629

Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. Nov 5; 98 (3)– Lonsdale DM, Thompson RD, Hodge TP. The integrated forms of the S1 and S2 DNA elements of maize male sterile mitochondrial DNA are flanked by a large repeated sequence. mitochondrial genomes were sequenced from highly purified mtDNA using the shotgun-sequencing method described previously for the NB maize mitochondrial genome (Clifton et al. ). The assemblies for NA, CMS-C, and CMS-T generated circular ‘‘master’’ ge-nomes that are depicted in linearized form in Figure 1.

Variation in mitochondrial translation products associated with male-sterile cytoplasms in maize. Proc Natl Acad Sci U S A. Aug; 75 (8)– [PMC free article] Dewey RE, Levings CS, 3rd, Timothy DH. Novel recombinations in the maize mitochondrial genome produce a unique transcriptional unit in the Texas male-sterile cytoplasm. Cell. Notably, only six seed mitochondrial phosphoproteins have been identified. Taking into account 64 phosphorylated mitochondrial proteins reported in plants to date, this amount is astonishingly low. One of the phosphorylated mitochondrial proteins observed in maize embryos is a small heat shock protein, HSP

A comparison of two-dimensional (2-D) polyacrylamide gel electrophoresis (PAGE) of proteins extracted from unstressed shoots and those that have been allowed to wilt shows that while many proteins are downregulated under water stress, many new proteins are synthesized (Fig. ).These results are confirmed by 2-D PAGE of in vitro-translated mRNAs extracted from stressed and . Next-generation sequencing (NGS) is used to test for the presence of mutations within the mitochondrial genome (includes 13 protein coding genes, 22 tRNA genes, and 2 rRNA genes). Large deletions within the mitochondrial genome are first detected by gel electrophoresis (as size-shifted PCR bands), and the locations of the deletions in the mtDNA.


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A comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis by David Walter Thornbury Download PDF EPUB FB2

Comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis Item Preview remove-circle Comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis by Thornbury, David Walter.

Publication date TopicsPages: Comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis Publisher: [Place of publication not identified] [publisher not identified] A Comparison of mitochondrial Proteins From Diverse Cytoplasms of Maize by Gel Electrophoresis By David Walter Thornbury June Chairman: Daryl R.

Pring Major Department: Plant Pathology Male sterility is a phenomenon of higher plants usually expressed as a failure to produce functional pollen.

Cytoplasmic male sterility. A comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis. By David Walter Thornbury. Abstract Mitochondria (lcsh), Hybrid corn (lcsh) Year: OAI identifier: oai:UFDC:AA_ Author: David Walter Thornbury.

A comparison of mitochondrial proteins from diverse cytoplasms of maize by gel electrophoresis / By David Walter Thornbury. Topics: Corn, Cytology, Disease and pest resistance, Dissertations, Academic, Genetic aspects, Hybrid corn, Mitochondria, Plant Pathology, Plant Pathology thesis Ph.

D, UF Hybrid corn, Mitochondria, Plant Author: David Walter Thornbury. Comparison of mitochondrial DNAs isolated from several male sterile rice cytoplasms Koh-ichi Kadowaki-1, Choyu Shinjyo-2 and Kyuya Harada 1) National Institute of Agrobiological Resources, Yatabe, Tsukuba Science City, Japan.

2) College of Agriculture, University of the Ryukyus, Nishihara, Okinawa, 01 Japan. The usefulness of the analysis of mitochondrial translation products as a method for classifying normal and male-sterile cytoplasms is discussed.

Discover the world's research 17+ million members. We have identified maize (Zea mays L. inbred B73) mitochondrial homologs of the Escherichia coli molecular chaperones DnaK (HSP70) and GroEL (cpn60) using two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblots.

During heat stress (42°C for 4 h), levels of HSP70 and cpn60 proteins did not change significantly. In contrast, levels of two kD proteins. Mitochondrial DNAs (mtDNAs) were isolated from 93 diverse races of maize from Latin America.

DNAs were examined by agarose gel electrophoresis of undigested DNA and. Analysis of 80S ribosomes of Arabidopsis (Arabidopsis thaliana) by use of high-speed centrifugation, sucrose gradient fractionation, one- and two-dimensional gel electrophoresis, liquid chromatography purification, and mass spectrometry (matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization) identified 74 ribosomal proteins (r-proteins), of which 73 are.

A comparison of the proteins identified via this maize mitochondrial proteome project to those identified via the analyses of the mitochondrial proteome of Arabidopsis (Kruft et al., ; Millar et al., ) and rice (Heazlewood et al., ) revealed that the mitochondrial proteomes of these species contain many similar proteins.

This was done CIASSIFICATION O F MAIZE CYTOPLASMS I - 70 - - - A d s 1c N S T C FIGURE.-Polyacrylamide gel rlectrophoresir of 1:olypeptides synthesized by mitochondria 1 from maize plants carrying 4 different types of cytoplasm in the same nuclear background (CO x WJ).Mitochondria wcre isolated from 4-day-old etiolated shoots and.

Gel electrophoresis of mitochondrial DNA from Ky21 demonstrated an approximately kb plasmid and no evidence for a kb plasmid found in many normal cytoplasms of North American lines of maize. A kb plasmid had been reported to be diagnostic for the T-type cms.

Mitochondria from S-type cytoplasmic male-sterile maize contain two small DNA species, S1 and S2, which are absent from other fertile and male-sterile cytoplasms. These species have been cloned in plasmid pBR by the homopolymer extension method.

Probes made with these recombinant plasmids have been used to establish the homology between high molecular weight mitochondrial DNAs of. Electrophoresis of mitochondrial proteins SDS/polyacrylamide slab gel electrophoresis of mitochondrial proteins Electrophoretic resolution of mitochondrial protein subunits was based on the methods described by Studier () using the discontinuous buffer.

Some plant cytoplasms express novel mitochondrial genes that cause male sterility. Nuclear genes that disrupt the accumulation of the corresponding mitochondrial gene products can restore fertility to such plants.

The Texas (T) cytoplasm mitochondrial genome of maize expresses a novel protein, URF13, which is necessary for T cytoplasm–induced male sterility. Comparison of the modern fertile maize mitochondrial genome (N) with an ancestral maize mitochondrial genome (RU) reveals a 12 kb duplication (containing the atpA gene) in the modern genome that is absent from the ancestor.

Cloning, mapping, and sequencing of the relevant portions of the ancestral genome shows that this duplication probably arose via a three-stage recombination. Plasmid-like mitochondrial DNA (mtDNA) associated with cytoplasmic male sterility (CMS) have been reported in some higher plants, e.g., maize, sorghum, sugarbeet, Brassica, Vicia faba, and sunflower.

InYamaguchi and Kakiuchi first discovered two plasmid-like mtDNAs, B-1 and B-2, associated with CMS in the BT type. INFLUENCE OF NUCLEAR BACKGROUND ON TRANSCRIPTION OF A MAIZE MITOCHONDRIAL REGION ASSOCIATED WITH TEXAS MALE STERILE CYTOPLASM Introduction The mitochondrial genome of the normal, male-fertile (N) cytoplasm of maize (Zea mays L.) is estimated to be kb (Lonsdale et al., ).

Approximately 42 kb of the maize mitochondrial genome is. Figure 1. NCS Mutants Express High Levels of AOX Protein. (A) Immunoblot analysis of mitochondrial proteins and AOX isolated from ear shoots.

Mitochondrial proteins (50 μg/lane) from ear shoots of NCS plants (lanes 2, 4, and 6, corresponding to NCS2, NCS4, and NCS6 samples, respectively) and their normal relatives (lanes 2N, 4N, and 6N) were loaded pairwise for comparison. Protein concentration is estimated by a standard protein assay (BCA or Bradford type).

Mitochondrial protein solubilization. Protein solubilization for two-dimensional gel electrophoresis is carried out the day of use by mixing at room temperature one volume of mitochondrial suspension, one volume of detergent.

Mitochondrial protein accumulation in developing N-cytoplasm pollen and CMS-S pollen carrying homozygous-viable restorer alleles. Proteins were immunodecorated and detected following denaturing gel electrophoresis.

Sample 1 contained 1 μg of protein extracted from N-cytoplasm Mo17 pollen mitochondria.PET1 and fertile cytoplasms. In organello protein synthesis experiments showed that the ca. 15 kDa mitochondrial polypeptide, characteristic of PET1, is not present in the CMS3 line.

These data suggest that the molecular basis of male sterility in the CMS 3 line differs from that of the PET 1 cytoplasm. The.